abstract：:An in situ assay for detecting DNA-binding proteins in herpes simplex virus type 1 (HSV-1)-infected cells is described. Seventeen HSV-induced DNA-binding species were visible with nicked, double-stranded DNA as a substrate, while fourteen virus-induced DNA-binding fractions were present in gels containing nuclease-tre...

journal_title：Acta virologica

authors： Mohammad F,Farber E

更新日期：1989-09-01 00:00:00

abstract：:Two batches of experimental media were prepared with Difco-Peptone and Centron-Peptone in combination with lactalbumin hydrolysate (LAH) in Eagle's salts containing 3 amino acids, vitamins and 1% bovine serum. Both medium batches supported the growth of Razi BHK-21 cells in serial passages and the replication of foot-...

journal_title：Acta virologica

authors： Saha SN,Sen AK

更新日期：1989-08-01 00:00:00

abstract：:Incubation of Aedes albopictus cells infected with Mayaro virus at 37 degrees C causes inhibition of virus replication. During the first hour post infection (p.i.) incubation at 37 degrees C inhibited cellular and virus proteosynthesis. A preferential translation of heat shock proteins 82 kD and 70 kD was observed. Af...

journal_title：Acta virologica

authors： Carvalho Mda G,Mezêncio Jda S,Rebello M,Freitas MS

更新日期：1989-05-01 00:00:00

abstract：:Sensitivity and specificity of a modified agar gel immuno-diffusion (AGID) test for the diagnosis of enzootic bovine leukosis was compared in 609 sera using the standard method, the modified test, and ELISA. The modifications concerned the composition of agar gel (1.8% Bacto-agar Difco), addition of polyethylene glyco...

journal_title：Acta virologica

authors： Wawrzkiewicz J,Dziedzic B,Kozioł T

更新日期：1989-03-01 00:00:00

abstract：:14C-labelled Lassa virus was purified by isopycnic centrifugation and split to subvirion fractions. The purified virus was treated with nonionic detergents Nonidet P-40 (NP-40) and with octylglycoside. After ultracentrifugation in urographin density gradient, two subvirion fractions with buoyant density of 1.24-1.26 a...

journal_title：Acta virologica

authors： Lemeshko NN,Gvelesjani IO,Ilkevich JS,Lukashevicp IL

更新日期：1989-03-01 00:00:00

abstract：:Total haemolytic complement levels were assessed in normal, fowl pox-vaccinated or infected chicks using radial immune haemolysis up to 28 days post-treatment. Significantly lower values of total haemolytic complement were recorded 7-21 days post-vaccination or 21 days post-infection as compared to controls (p less th...

journal_title：Acta virologica

authors： Kapil S,Das SK,Kumar A

更新日期：1989-01-01 00:00:00

abstract：:Antigenic relationships between the four subgroups of the Kemerovo serogroup of tick-borne orbiviruses were examined. Kemerovo (KEM subgroup), Broadhaven (BRD) and Wexford (WEX) [Great Island (GI) sub-group], Chenuda (CNU sub-group), and Wad Medani (WM sub-group) viruses cross-reacted in immunofluorescence tests. Comp...

journal_title：Acta virologica

authors： Carey D,Nuttall PA

更新日期：1989-01-01 00:00:00

abstract：:The effect of some cellular function inhibitors on adsorption and successive events of penetration of vesicular stomatitis virus to phylogenetically unrelated permissive cells was investigated. Treatment of HeLa, CER, EPC and Aedes albopictus cells with colcemide and cytochalasin D which affect cytoskeleton organizati...

journal_title：Acta virologica

authors： Superti F,Seganti L,Orsi N

更新日期：1988-11-01 00:00:00

abstract：:The dsRNA profiles of the Czechoslovakian and Siberian serotypes of the Kemerovo serocomplex viruses examined were similar in agarose, while their dsRNA profiles were distinct in polyacrylamide gel. Blot hybridization studies of the Kemerovo serocomplex viruses demonstrated that the genes were highly conserved among t...

journal_title：Acta virologica

authors： Brown SE,Morrison HG,Buckley SM,Shope RE,Knudson DL

更新日期：1988-09-01 00:00:00

abstract：:Herpes simplex virus (HSV) DNA was found by spot blot hybridization in the right nc. amygdalae of 3 out 10 patients who underwent curative stereotactic surgery for severe mental retardation with aggressive behavior and/or paranoic schizophrenia. Of these, 6 were also tested for the presence of CMV DNA sequences with n...

journal_title：Acta virologica

authors： Kúdelová M,Rajcáni J,Pogády J,Sramka M

更新日期：1988-09-01 00:00:00

abstract：:The level of the Epstein-Barr virus (EBV)-determined nuclear antigen (EBNA-1) encoded by the Bam HI-K fragment of EBV DNA remained unchanged in P3HR-1 cells after induction of the productive cycle of virus replication by sodium-n-butyrate and 12-o-tetradecanoylphorbol-13-acetate (TPA). This was shown by the same capac...

journal_title：Acta virologica

authors： Nováková Z,Krchnák V,Roubal J

更新日期：1988-07-01 00:00:00

abstract：:We isolated plaque-size variants of a South American strain of yellow fever virus, and compared their ability to infect orally and be transmitted by vector Aedes aegypti mosquitoes with that of the uncloned, parental virus. We analyzed the same clonal isolates in mouse virulence experiments. No significant differences...

journal_title：Acta virologica

authors： Miller BR,Adkins D

更新日期：1988-05-01 00:00:00

abstract：:Variation of antibody level against West Nile (WN) virus depending on the season of birth was followed among 151 paired serum samples of healthy Pakistani persons in Karachi, collected twice in July and October, 1985. The persons born during the months between February and June had lower positive antibody rate and low...

journal_title：Acta virologica

authors： Sugamata M

更新日期：1988-03-01 00:00:00

abstract：:Thirty four chemical compounds were injected into rabies infected mice by intramuscular (i.m.) route. Twenty four compounds such as well known therapeutic agents: amantadine, lipacids, phenol compounds, didemnin-B, procaine, nucleosides analogues (ribavirin, tiazofurin, pyrazofurin) had no effect. Two compounds had a ...

journal_title：Acta virologica

authors： Bussereau F,Picard M,Blancou J,Sureau P

更新日期：1988-01-01 00:00:00

abstract：:An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibodies against equine herpesvirus type 2 (EHV-2) in equine sera. The optimal conditions of antigen concentration, and serum and conjugate dilutions were established by chequerboard titrations. When the standard ELISA test was ...

journal_title：Acta virologica

authors： Fu ZF,Denby L,Lien DH,Robinson AJ

更新日期：1987-11-01 00:00:00

abstract：:Transfer factor specific for herpes simplex virus (HSV) type 1 (TFHSV-1) was prepared from splenic cells of HSV-1 immunized mice. Protection was transferred with TFHSV-1 to nonimmune mouse recipients. The TFHSV-1 injected mice had a higher survival rate after lethal HSV-1 challenge as compared to mice injected with a ...

journal_title：Acta virologica

authors： Huang LL,Su CZ,Wan ZF

更新日期：1987-11-01 00:00:00

abstract：:Results of the direct immunofluorescence (IF) test in 152 clinical specimens (throat swabs) were compared with those of respiratory syncytial virus (RSV) isolation. The prevalence of RSV infections in the upper and lower respiratory tract was high especially in infants until 12 months of age. The average RSV isolation...

journal_title：Acta virologica

authors： Mlinarić-Galinović G,Ugrcić I,Cvetković M,Pende B,Ivanković D

更新日期：1987-09-01 00:00:00

abstract：:A mathematical model of the pathogenesis of experimental Mengo virus infection in mice has been developed and fitted using kinetic data of both virus multiplication in different organs and mortality. The behaviour of the model proved to be bistable. In contrast to the widely accepted hypothesis that an acutely virus-i...

journal_title：Acta virologica

authors： Guthke R,Veckenstedt A,Güttner J,Stracke R,Bergter F

更新日期：1987-08-01 00:00:00

abstract：:Different concentrations of chemically-treated (by potassium periodate oxidation or mild acid hydrolysis) purified phase I Coxiella burnetii (C.b.) corpuscles and natural (untreated) purified phase I and phase II C.b. corpuscles were compared by ELISA for detection of both phase I (directed to antigen 1) and phase II ...

journal_title：Acta virologica

authors： Kovácová E,Gallo J,Schramek S,Kazár J,Brezina R

更新日期：1987-05-01 00:00:00

abstract：:The presence and the level of hepatitis B surface antigen (HBsAg)/IgM complexes were determined in 54 chronic HBsAg carriers in relation to receptors for polymerized human serum albumin (pHSA-R) tested by specific radioimmunoassay, and to hepatitis B virus-DNA polymerase (HBV-DNAp). HBsAg/IgM complexes, correlated sig...

journal_title：Acta virologica

authors： Mora I,Carreño V,Porres JC,Gutiez J,Guio CH

更新日期：1987-03-01 00:00:00

abstract：:2-0-hexadecyl-glycero-3-phosphocholine (GPC) 1 mmol/l solution reduced the infectivity of red clover mottle virus (RCMV) by over 90%. The binding of GPC molecules to RCMV particles was investigated with 1H-nmr spectroscopy, which showed a striking interaction between virus particles and GPC molecules. In 10 mmol/l GPC...

journal_title：Acta virologica

authors： Kluge S,Gawrisch K,Nuhn P

更新日期：1987-03-01 00:00:00

abstract：:Experimental parenteral inoculation of Dermacentor reticulatus ticks with the rickettsia Coxiella burnetii (C.b.) and tick-borne encephalitis (TBE) virus resulted in a generalized rickettsial and viral infection, irrespective of whether the agents were given simultaneously or by a 7 days interval apart. Both agents mu...

journal_title：Acta virologica

authors： Rehácek J,Kovácová E,Ciampor F,Gresíková M,Tarasevich IV

更新日期：1987-01-01 00:00:00

abstract：:Protective effects of different types of Q fever vaccines, namely untreated Coxiella burnetii phase I cells (Cb I) or Cb I cells treated with chloroform-methanol (CM) mixture (Cb I-CM) and of a Q fever chemovaccine obtained by trichloroacetic acid extraction (TCAE) from intact Cb I cells, were compared in mice and gui...

journal_title：Acta virologica

authors： Kazár J,Votruba D,Propper P,Schramek S

更新日期：1986-11-01 00:00:00

abstract：:Genetic variation in Japanese encephalitis (JE) virus strains after various passage histories was examined by oligonucleotide fingerprints of the 42S genome RNA. The results indicated that genome changes occurred during passages of new virus isolates through cell lines or mouse brains, especially at earlier passage le...

journal_title：Acta virologica

authors： Hori H,Igarashi A,Yoshida I,Takagi M

更新日期：1986-09-01 00:00:00

abstract：:Substance P, a neuropeptide of the tachykinin group, inhibits measles virus replication in cell culture and partially blocks viral fusion activity assayed in the haemolysis system. The ID50 for the inhibition of measles virus single-cycle replication is 0.6 mumol/l, and the effect is fully reversible. The antiviral ac...

journal_title：Acta virologica

authors： Schroeder C

更新日期：1986-09-01 00:00:00

abstract：:The rate of the synthesis of tick-borne encephalitis (TBE) virus-specified proteins at high multiplicity of infection (MOI of 100 PFU per cell) was the highest by 8-14 hr post-infection (p.i.) as compared to the lower MOI of 4 PFU per cell (14 to 17 hr p.i.). The first virus-specific proteins appeared in cells from 2 ...

journal_title：Acta virologica

authors： Lyapustin VN,Gritsun TS,Lashkevich VA

更新日期：1986-07-01 00:00:00

abstract：:A collection of 39 influenza A virus strains of the subtype H3N2 isolated in G.D.R. and of six reference strains were analysed with regard to the antigenic structure of their surface proteins haemagglutinin (HA) and neuraminidase (NA) as well as regarding their polypeptide variations. For the field strains during the ...

journal_title：Acta virologica

authors： Seidel W,Herrmann B,Langhammer I,Geisler B,Döhner L,Ghendon Yu

更新日期：1986-05-01 00:00:00

abstract：:In result of 20 pregnancies complicated by varicella 11 healthy and 6 defective offspring were born, 2 pregnancies were aborted by the physician and one pregnancy terminated by stillbirth of 3 siblings. Laboratory investigation of 16 pregnancies has shown that transplacental transfer of varicella-zoster (VZ) virus did...

journal_title：Acta virologica

authors： Trlifajová J,Benda R,Benes C

更新日期：1986-05-01 00:00:00

abstract：:Interferon (IFN) was induced in chicken peripheral blood leukocytes by exposure to Concanavalin A (Con A). It has reached a maximum level 96 hr after stimulation with the optimum dose of 10 micrograms/ml. The IFN was partially purified by chromatography on controlled-pore glass adsorbent and was characterized as a fai...

journal_title：Acta virologica

authors： Pusztai R,Taródi B,Béládi I

更新日期：1986-03-01 00:00:00

abstract：:Current isolates of the subtypes H7N3 and H7N7 from 1979 to 1981 were examined and compared with the reference strains with regard to their antigenic variability and to their pathogenicity for birds and mammals in order to establish the potentiality of influenza A/H7 virus (Hav1) transmission from birds to mammals. Th...

journal_title：Acta virologica

authors： Sinnecker H,Egert J,Eggert HJ,Gogolin J,Gümpel S,Schrader C,Sinnecker R,Zilske E

更新日期：1986-01-01 00:00:00

abstract：:Neonatal rat heart muscle primary cell cultures were infected with influenza A viruses. Depending on the infectious dose, functional and structural changes of the heart muscle cells could be registered. The system seems suitable for testing the cytotoxicity of different influenza virus strains. ...

journal_title：Acta virologica

authors： Suciu EM,Muschner K,Witzleb W

更新日期：1985-12-01 00:00:00

abstract：:Chromic chloride and tannic acid methods were elaborated to bind purified adenovirus hexons to sheep red blood cells (SRBC). Either method gave very sensitive haemagglutination (HA) with adenovirus antisera, but treatment with tannic acid was more sensitive. Using this method, specific antibodies present in polyclonal...

journal_title：Acta virologica

authors： Lengyel A,Adám E,Nász I,Erdei J,Fachet J

更新日期：1985-09-01 00:00:00

abstract：:Although most pathogenic rickettsiae are obligate intracellular parasites, it is clear that they are bacteria. As such, form and function in rickettsiae are closely similar to form and function found in their free-living counterparts. This review of rickettsiae as bacteria portrays the broad similarities of rickettsia...

journal_title：Acta virologica

authors： Ormsbee RA

更新日期：1985-09-01 00:00:00

abstract：:Over a 5-year observation period in a children-institution community, the severity of respiratory-syncytial (RS) virus infection varied within a broad range depending on biological properties of the circulating virus population. The type of virus population was characterized by a complex of biological features, namely...

journal_title：Acta virologica

authors： Yurlova TJ,Zoshchenkova NYa,Karpova LS

更新日期：1985-07-01 00:00:00

abstract：:The achievements and perspectives of genetic manipulations are described aiming at preparation of first generation subunit vaccines based on the synthesis in bacterial and eukaryotic cells of full-sized virion proteins expressing the main antigenic determinants. The preparation of such vaccines in bacterial cells seem...

journal_title：Acta virologica

authors： Tickhonenko TI

更新日期：1985-05-01 00:00:00

abstract：:In vivo phagocytosis activity of macrophages (PAM) was temporarily suppressed in mice by application of a suspension of microscopic from particles. As demonstrated, a reversible block of 70% of PAM was accompanied by a marked increase of the lethality during the acute tick-borne encephalitis (TBE) virus infection. Asy...

journal_title：Acta virologica

authors： Khozinsky VV,Semenov BF,Gresíková M,Chunikhin SP,Sekeyová M,Kozuch O

更新日期：1985-05-01 00:00:00

abstract：:The sensitivity of enzyme linked immunoassay (ELISA) was compared to that of haemagglutination inhibition (HI) test in detection of humoral antibodies in birds vaccinated with Newcastle disease virus (NDV). The highest antibody titre at day 21 post-vaccination as detected by ELISA in the sera of vaccinated birds was 2...

journal_title：Acta virologica

authors： Mishra SC,Rai A,Jaiswal TN

更新日期：1985-03-01 00:00:00

abstract：:It has been shown that serum blocking factor(s) (SBF) previously detected in normal mice bind(s) to receptors on the surface membranes of virus-induced autoreactive T lymphocytes (ARTL) and to that of ARTL activity inhibiting T suppressors (TSar). The interaction of SBF with the receptors is reversible, H-2 restricted...

journal_title：Acta virologica

authors： Khozinsky VV,Semenov BF

更新日期：1985-01-01 00:00:00

abstract：:In order to determine the definitive importance of T- and B-lymphocytes in immunity to Rickettsia conorii, mice genetically deficient in T-cells, B-cells, or both T- and B-cells were infected experimentally. T-lymphocytes rather than humoral antibodies were crucial to rickettsial clearance and a reduced mortality rate...

journal_title：Acta virologica

authors： Montenegro NR,Walker DH,Hegarty BC

更新日期：1984-11-01 00:00:00

abstract：:Human influenza virus A/Krasnodar/101/59 (H2N2) was passaged in chick fibroblast cultures in the presence of trypsin at suboptimal temperature. The virus which underwent 16 passages at 28 degrees C possessed cold-adapted (ca) and temperature sensitive (ts) phenotypes and formed larger plaques at the optimal temperatur...

journal_title：Acta virologica

authors： Panzig B,Döhner L,Lisovskaya KB,Ghendon YZ

更新日期：1984-09-01 00:00:00